Foraging behavior of Aphidius matricariae (Hymenoptera: Braconidae) on tobacco aphid, Myzus persicae nicotianae (Hemiptera: Aphididae).

The aim of this study was to investigate the foraging behavior of Aphidius matricariae (Haliday) (Hymenoptera: Braconidae) as a biological control agent of Myzus persicae nicotianae Blackman (Hemiptera: Aphididae), a key and cosmopolitan pest of tobacco fields. To achieve a strategy for the control of this pest and a mass-rearing program of the parasitoid, host stage preference, switching, functional response, and mutual interference of A. matricariae were investigated at 25 ± 1°C, 70 ± 5% RH and 16:8 h L:D photoperiod. The parasitoid showed a preference for third- and fourth-instar nymphs of tobacco aphid in both choice and no-choice experiments. Using the Murdach's model, switching behavior was observed in A. matricariae between different density proportions of third- and fourth-instar nymphs. Further, the parasitoid exhibited a type II functional response when it was offered to third-instar nymphs of M. persicae nicotianae at six densities (2, 4, 8, 16, 32, and 64). Based on the linear regression analysis, there was a significance difference between the logarithm of per capita searching efficiency and the logarithm of parasitoid density. As the wasp density increased, per capita searching efficiency decreased. The result of this study revealed that A. matricariae is an effective agent in the integrated management of M. persicae nicotianae. In addition, application of these results can be important in mass-rearing program of A. matricariae.

Effects of plant availability on population size and dynamics of an insect community: diamondback moth and two of its parasitoids.

To understand the effect of plant availability/structure on the population size and dynamics of insects, a specialist herbivore in the presence of two of its parasitoids was studied in four replicated time-series experiments with high and low plant availabilities; under the latter condition, the herbivore suffered from some periods of resource limitation (starvation) and little plant-related structural refuges. Population dynamics of the parasitoid Cotesia vestalis was governed mainly by the delayed density-dependent process under both plant setups. The parasitoid, Diadegma semiclausum, under different plant availabilities and different coexistence situations (either +competitor or -competitor) showed dynamics patterns that were governed mainly by the delayed density process (significant lags at weeks 2-4). Both the competing parasitoids did not experience beneficial or costly interferences from each other in terms of their own population size when the plant resource was limited. Variation in the Plutella xylostella population under limited plant availability is higher than that under the other plant setup. For both parasitoids, under limited plant setup, the extinction risk was lower when parasitoids were engaged in competition, while under the unlimited plant setup, the mentioned risk was higher when parasitoids competed. In this situation, parasitoids suffered from two forces, competition and higher escaped hosts.

Biological study of Plutella xylostella (L.) (Lep: Plutellidae) and it's solitary endoparasitoid, Cotesia vestalis (Haliday) (Hym. Braconidae) under laboratory conditions.

Plutella xylostella (L.) (Lep: Plutellidae), is a destructive pest of brassicaceous crops in the world. Cotesia vestalis (Haliday) is one of most important biological control agents of P. xylostella in the world and Iran. Both of P. xylostella and C. vestalis biology were carried out in laboratory condition. Results showed that development time of immature stages of P. xylostella including egg, Instar I, Instar II, Instar III, Instar IV, prepupa, pupa were 2.39 +/- 0.17, 2.18 +/- 0.17, 2.06 +/- 0.28, 2.14 +/- 0.14, 2.54 +/- 0.12, 0.40 +/- 0.12 and 4.23 +/- 0.23 days, respectively. Longevity of female and male were 28.26 +/- 0.05 and 30.22 +/- 0.05 days. By dissecting the parasitized larvae, the egg incubation period of C. vestalis was recorded 1.73 +/- 0.06 days. In long-term oviposition trials, females laid eggs on P. xylostella larvae for up to 10 days. Larval development of the parasitoid in host only required 6.47 days: the first instar larva required 3.25 +/- 0.047 days; the second instar larva needed 2.78 +/- 0.1 days and the third instar larvae exited the host and pupated in, 0.4 +/- 0.07 days. Prepupal and pupal period of wasp were 1.9 +/- 0.06 and 2.13 +/- 0.09 day, respectively. Unmated female and male longevity of wasp were 16.83 +/- 0.37, 16.25 +/- 0.17 and sex ratio is male-biased. When a mixed group and isolated of instars were presented for parasitoid, the 2nd and 3rd instar larvae were so preferred and the 4th instar was less attractive for selection. In choice experiment, the percentage parasitism of 2nd, 3rd and 4th instars was 78.58, 69.94 and 4.36%, respectively. The rapid oviposition rate, short life duration and high percentage parasitism increases parasitoid potential for suppression of host population. Present results suggest that C. vestalis has considerable potential as a biological control agent for P. xylostella.